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HPLC Enantioseparation on Cyclodextrin-Based Chiral Stationary Phases

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Cyclodextrin-bonded silica material is one of the most commonly used chiral stationary phases in liquid chromatography for pharmaceutical analysis and enantioseparations. The approaches for immobilization of cyclodextrins onto the silica surface influence both, the stability of the chiral stationary phase and the enantioselectivity. In this chapter, we describe an established example of the preparation of a cyclodextrin-based chiral stationary phase via “click chemistry” and their application for enantioseparations of racemic compounds by HPLC.
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