Preparation and Solubilization of Body Fluids for 2-D

Cells, tissues, and organs require energy to function normally. Some of them are metabolic providers, and some others are users. Body fluids act as transporters and distributors of metabolic “fuels” between cells and maintain relatively constant conditions through homeostasis. The ratio of solute to water in all body fluids is maintained within a very narrow range. The concentrations of body fluid metabolites are thus extremely well regulated. Many diseases or environment disturbances lead to changes in the expression of body fluid proteins. Information on these changes is often of diagnostic, prognostic, or therapeutic importance.