PROTOCOL TO EXTRACT DNA FROM PARAFFIN BLOCKS
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PROTOCOL TO EXTRACT DNA FROM PARAFFIN BLOCKS
Solutions
Digestion buffer : 100 mM NaCl/10mM Tris-HCl, pH 8.0 and 25 mM EDTA, pH 8.0/0.5% SDS. Store at RT.
Proteinase K: Stored as 20 mg/ml aliquots at -20 C; Can be refrozen a few times.
Use Proteinase K at 0.3-0.5 mg/ml in digestion buffer
PCI : phenol/chloroform/isoamyl alcohol (from Ameresco)
