【资源】DNA 纯化的问题及解决（很不错的）

DNA Purification

Sibylle Herzer

内容:

What Criteria Could You Consider When Selecting a
Purification Strategy? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 168
How Much Purity Does Your Application Require?. . . . . . . . 168
How Much Nucleic Acid Can Be Produced from a Given
Amount of Starting Material? . . . . . . . . . . . . . . . . . . . . . . . . 168
Do You Require High Molecular Weight Material? . . . . . . . . 168
How Important Is Speed to Your Situation? . . . . . . . . . . . . . 168
How Important Is Cost? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169
How Important Is Reproducibility (Robustness) of the
Procedure? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169
What Interferes with Nucleic Acid Purification? . . . . . . . . . . 169
What Practices Will Maximize the Quality of DNA
Purification?. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171
How Can You Maximize the Storage Life of Purified
DNA?. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172
Isolating DNA from Cells and Tissue . . . . . . . . . . . . . . . . . . . . . 172
What Are the Fundamental Steps of DNA
Purification?. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172
What Are the Strengths and Limitations of Contemporary
Purification Methods? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 174
What Are the Steps of Plasmid Purification? . . . . . . . . . . . . . 180
What Are the Options for Purification after In Vitro
Reactions? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 184
Spun Column Chromatography through Gel Filtration
Resins. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 184
Filter Cartridges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185
Silica Resin-Based Strategies . . . . . . . . . . . . . . . . . . . . . . . . . . . 186
Isolation from Electrophoresis Gels . . . . . . . . . . . . . . . . . . . . 187
What Are Your Options for Monitoring the Quality of
Your DNA Preparation? . . . . . . . . . . . . . . . . . . . . . . . . . . . . 190
Bibliography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 191