CD49a/CD49b/CD49c/CD49d/CD49e/CD49f 分子
CD49a
常用单克隆抗体或代号： SR84,IB3.1;(VLAa 1)
主要表达细胞： Ta,Ba,M [AS]
分子质量(kDa)和结构： gp210,与CD29组成VLA- 1
功 能： 粘附CA和LM
CD49b
常用单克隆抗体或代号： Gi94;(VLA- a 2,ECMR- Ⅱ,Pt- GPIa)
主要表达细胞： Leu,Pt,Fb,En [AS]
分子质量(kDa)和结构： gp160,与CD29组成VLA- 2
功 能： 粘附CA、LM,人肠道细胞病变孤病毒1(ECHO病毒1)受体
CD49c
常用单克隆抗体或代号： J143;(VAL- a 3,ECMR- I)
主要表达细胞： T,Bsub,M [AS]
分子质量(kDa)和结构： gp150与CD29组成VAL- 3
功 能： 粘附FN、CA和LM
CD49d
常用单克隆抗体或代号： B5G10,HP2/1;(VLA- a 4,LPAM)
主要表达细胞： M,T,B,Thy,Pt [AS]
分子质量(kDa)和结构： gp150,与CD29组成VLA- 4,与b 7组成a 4/b 7为MadCAM- 1受体
功 能： 粘附FN,结合VCAM- 1(CD106),归位受体,T- B细胞粘附
CD49e
常用单克隆抗体或代号： 2H6,3D3;(VLAa 5,FNRa ,ECMR- Ⅳ)
主要表达细胞： Pt,T,PMN,Bsub,M[AS]
分子质量(kDa)和结构： gp160(135/25二硫键连接,与CD29组成VLA- 5
功 能： 粘附FN
CD49f
常用单克隆抗体或代号： GOH3;(VAL- a 6,血小板gplc’)
主要表达细胞： Pt,Meg,Tsub [AS]
分子质量(kDa)和结构： gp150(120/30二硫键连接,与CD29组成VLA- 6
功 能： 粘附LM
CD49a
Aka very late antigen (VLA) alpha 1 chain, integrin alpha 1 chain
Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins.
Very late activation proteins (VLA) are a family of integrins originally identified on activated human T cells, later on fibroblasts, platelets, others. Six forms, VLA1 to VLA6, have been identified, each with distinct alpha chain (numbered alpha-1 to alpha-6) associated with a common beta chain.
CD49a - Laminin and collagen receptor
Positive staining (normal): activated B and T cells, monocytes
CD49b
Aka very late antigen (VLA) alpha 2 chain - on T cells
Aka GPIa/IIa when expressed on platelets
Receptor for laminin, collagen, fibronectin, e-cadherin
Responsible for platelet adhesion to collagen (blood clotting), role in angiogenesis
Positive staining (normal): platelets, activated B & T cells
CD49c
Aka very late antigen (VLA) alpha 3 chain
Receptor for laminin, collagen, fibronectin, thrombospondin
Positive staining (normal): nonhemaoietic cells
CD49d
Aka very late antigen (VLA) alpha 4 chain
Receptor for fibronectin, thrombospondin, VCAM-1
Role in cell-cell interactions and cell adhesion to the extracellular matrix
Promotes (a) lymphocyte migration into tissue by strengthening lymphocyte adhesion to endothelial cells, (b) rolling of T cells in vascular lumen on VCAM-1 of endothelium, (c) homing of T cell subsets to Peyers patches, (d) differentiation of hemaoietic precursor cells by adhesion to bone marrow stromal cells
Relevant to tumor progression and metastasis
Positive staining (normal): macrophages, T and B cells, thymocytes, eosinophils, basophils, NK cells, dendritic cells, myeloid cells, erythrocyte precursors
Positive staining (disease): melanoma cells
Negative staining: erythrocytes, platelets, neutrophils
CD49e
Aka very late antigen (VLA) alpha 5 chain
Fibronectin and fibrinogen receptor
Positive staining (normal): platelets, monocytes, neutrophils
CD49f
Aka very late antigen (VLA) alpha 6 chain
Laminin receptor
Important for formation of hemidesmosomes of stratified squamous and transitional epithelia
Positive staining (normal): epithelial cells
