Accurate Measurement of Nicotinamide Adenine Dinucleotide (NAD+) with High-Performance Liquid Chromatography

Nicotinamide adenine dinucleotide (NAD⁺ ) plays a critical role in regulating numerous biological and physiological pathways including metabolism, inflammation, cancer, and aging in mammals. Here we describe a highly quantitative method with reverse-phase high-performance liquid chromatography (HPLC) for the determination of NAD⁺ levels in cells and tissues. This methodology provides accurate, reliable, and reproducible results of NAD⁺ measurement, which enables us to analyze various pathophysiological changes in NAD⁺ levels in vitro and in vivo.