Differential Detergent Fractionation of Eukaryotic Cells: Analysis by Two-Dimensional Gel Electrophoresis
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Enhance the delectability of low-abundance species or semipurify components of known subcellular localization.
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Define the subcellular localization of enzymes, regulatory, or structural proteins as well as nonprotein metabolites.
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Monitor physiologic fluxes and compartmental redistribution of biomolecules under basal and stimulated conditions.
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Identify cytoskeletal-associated and interacting proteins.
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Investigate the role of cytoskeletal networks in the subcellular localization of endogenous and exogenous factors, including mRNA, viral components, and heat shock proteins, interactions relevant to understanding mechanisms of infection, protein turnover, and the stress response.
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