Intrabody Expression in Eukaryotic Cells

互联网2014-02-13

404

We describe procedures for intracellular expression of scFv in eukaryotic cells. Starting from a scFv gene cloned in a phage-display vector, we describe the cloning step into a mammalian expression vector, the transient transfection of a HeLa cell line, and the monitoring of intrabody expression by immunofluorescence staining and FACS analysis.

相关产品推荐

SCGB1A1,CC10,CCSP,UGB/SCGB1A1,CC10,CCSP,UGB蛋白Recombinant Human Uteroglobin protein (SCGB1A1) (Active)重组蛋白Clara cell phospholipid-binding protein, Clara cells 10 kDa secretory protein, CC10, Secretoglobin family 1A member 1, Urinary protein 1蛋白

￥852

Scgb1a1,Cc10,Ugb,Utg/Scgb1a1,Cc10,Ugb,Utg蛋白Recombinant Rat Uteroglobin protein (Scgb1a1) (Active)重组蛋白Clara cell phospholipid-binding protein, Clara cells 10 kDa secretory protein, CC10, PCB-binding protein, Secretoglobin family 1A member 1蛋白

￥852

XPC/XPC蛋白Recombinant Human DNA repair protein complementing XP-C cells (XPC)重组蛋白Xeroderma pigmentosum group C-complementing proteinp125蛋白

￥1344

Trem2/Trem2蛋白/Short name: TREM-2 Alternative name(s): Triggering receptor expressed on monocytes 2蛋白/Recombinant Mouse Triggering receptor expressed on myeloid cells 2 (Trem2), partial重组蛋白

￥69

AQP1/AQP1蛋白/Aquaporin-CHIPUrine water channelWater channel protein for red blood cells and kidney proximal tubule蛋白/Recombinant Human Aquaporin-1 (AQP1), partial重组蛋白

￥69