Recovery of Human Mycoplasmas
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There are a range of methods that can be used to recover mycoplasmas from the infected sites of humans. The current standard method is culture on supplemented agar or broth media. The prolonged time for culture particularly for Mycoplasma pneumoniae , has necessitated a reliance by diagnostic laboratories on serological techniques that are beyond the scope of this text but are summarized in Table 1 .
Table 1 An Evaluation of Serological Tests for M. Pneumoniae a
|
Assay |
Sensitivity |
Specificity |
Speed |
Commercially available |
Cost |
|---|---|---|---|---|---|
|
Complement fixation test |
++ |
++ |
18 h |
Not in total |
+ |
|
Cold agglutinins |
++ |
+ |
4 h |
N |
+ |
|
Immunofluorescent antibody |
+++ |
++ |
3 h |
Y |
++ |
|
Indirect agglutination |
+++ |
++ |
4 h |
Y |
+ |
|
μ ELISA IgG |
++ |
++ |
4 h |
Y |
++ |
|
ELISA IgM (τ capture) |
+++ |
+++ |
4 h |
Y |
++ |
a + = Low, ++ = moderate, +++ = high.
