Agarose Gel Electrophoresis of RNA
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实验试剂
NorthernMax Formaldehyde Load Dye
NorthernMax-Gly Sample Loading Dye
实验步骤
1) Heat 1 g agarose in 72 ml water until dissolved, then cool to 60°C.
2) Add 10 ml 10X MOPS running buffer, and 18 ml 37% formaldehyde (12.3 M).
3) Pour the gel using a comb that will form wells large enough to accommodate at least 25 µl.
1) To 1-3 µg RNA, add 0.5-3X volumes Formaldehyde Load Dye.