DIG Southern Blot Solutions
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1400
0.25M HCl (1L)
- 20.66mL HCl
- 979.34mL dH2 O
- ~undefinedadd acid to wateundefined
1M ammonium acetate, 0.02M NaOH (1L)
- 77.08g ammonium acetate
- 0.8g NaOH
- Fill with dH2 O
0.5X SSC, 0.1% SDS (1L)
- 25mL 20X SSC
- 10mL 10% SDS
- 965mL dH2 O
10X Blocking Buffer Þ 1X (30mL)
- 3mL Blocking Buffer stock
- 27mL Maleic Acid Buffer
Wash Buffer 3 (30mL)
- 30mL Maleic Acid Buffer
- 90µ l Tween 20 (cut off pipet tip)
Detection Buffer (1L)
- 100mL 1M Tris pH 9.5
- 20mL 5M NaCl
- 880mL dH2 O
- ~undefinedAutoclavundefined
1.5M NaCl, 0.5M NaOH (1L)
- 87.66g NaCl
- 20g NaOH
- Fill with dH2 O
2X SSC, 0.1% SDS (1L)
- 100mL 20X SSC
- 10mL 10% SDS
- 890mL dH2 O
Maleic Acid Buffer (1L)
- 11.61g Maleic Acid
- 8.766g NaCl
- Fill with dH2 O
- ~undefinedcheck pH 7.5 with NaOH pellets (~7g)
- ~undefinedAutoclavundefined
Diluting Ab 1:10,000 (30mL)
- 3mL Blocking Buffer stock
- 27mL Maleic Acid Buffer
- 3µ l Ab
