【共享】【实验室新手必看】分子生物及基因工程实验操作视频(可下载及在线观看)
丁香园论坛
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此资源转自小木虫,都可以下,相当清晰,但是需要安装米人,本人测试过了。希望好心的版主可以给点积分,谢谢了先
对于刚进实验室的新手来说,有太多仪器不懂,有太多操作不了解,现在把浙大基因工程实验操作讲解视频分享给大家,希望能对实验室新手有帮助~
1.细菌基因组DNA的提取.wmv
http://d.namipan.com/d/ba51df852bf4755d02e6a74a8ef3e04000f112bd57a77f04
2.PCR技术.wmv
http://d.namipan.com/d/12b239146091fdb90b0c3b71cce3ef3dd186339a039a7701
3.PCR产物的提纯.wmv
http://d.namipan.com/d/7c9f129943997592dfd4c72ca571418fafe6c3f39d239001
4.DNA的Southern转移.wmv
http://d.namipan.com/d/0088f3174c8b1c5fb9552f210e33754b173aad895b1e0902
5.表达产物的(sds-page)电泳鉴定.wmv
http://d.namipan.com/d/591ba7000ce0d8280442b351416593e17d97bc6f1be98304
6.分子杂交.wmv
http://d.namipan.com/d/6a9f006f9a9bb6affb3e7cdf56e94a0f305dfbc585f4d001
7.感受态细胞的制备.wmv
http://d.namipan.com/d/2053ba246d2f661a17cf52da46a5311c8f857fe8c779d501
8.DNA片段的体外连接.wmv
http://d.namipan.com/d/101d056b43b4e1343912dcfd161246529ccf381eadf6e500
9.探针的标记.wmv
http://d.namipan.com/d/6e3aea7410b7376173895798353d57fa05b50310cd550501
10.探针的检测.wmv
http://d.namipan.com/d/e024fad4e98fcf64a8f877c44aa035d8319a64ed0bfe1801
11.重组DNA的转化.wmv
http://d.namipan.com/d/682ae36c71aa2890bc37ce6dcb3bf8c67d78abf4e70fc702
12.重组子的选择.wmv
http://d.namipan.com/d/b0409400fe56f48ccf832b8bec061a4f1ce5877d45a17b01
13.载体质粒的制备及鉴定.wmv
http://d.namipan.com/d/00887d613029b3be029d95c6977980dfe021decc29d03503
14.目的基因和质粒DNA的限制性内切酶酶切反应.wmv
http://d.namipan.com/d/c256107edcc4ab2d3531e342faa34d298dfb62f8f3193e01
15.外源基因在宿主菌中的诱导表达.wmv
http://d.namipan.com/d/9aa3e5314e8b02c9fca0f6d65669c2c8978a73237df3bb02
16.染色体DNA的酶切与电泳鉴定.wmv
http://d.namipan.com/d/52f224fe9c9d0bb99318c5fa98ebcfc05884eb78c5dd2d01
对于刚进实验室的新手来说,有太多仪器不懂,有太多操作不了解,现在把浙大基因工程实验操作讲解视频分享给大家,希望能对实验室新手有帮助~
1.细菌基因组DNA的提取.wmv
http://d.namipan.com/d/ba51df852bf4755d02e6a74a8ef3e04000f112bd57a77f04
2.PCR技术.wmv
http://d.namipan.com/d/12b239146091fdb90b0c3b71cce3ef3dd186339a039a7701
3.PCR产物的提纯.wmv
http://d.namipan.com/d/7c9f129943997592dfd4c72ca571418fafe6c3f39d239001
4.DNA的Southern转移.wmv
http://d.namipan.com/d/0088f3174c8b1c5fb9552f210e33754b173aad895b1e0902
5.表达产物的(sds-page)电泳鉴定.wmv
http://d.namipan.com/d/591ba7000ce0d8280442b351416593e17d97bc6f1be98304
6.分子杂交.wmv
http://d.namipan.com/d/6a9f006f9a9bb6affb3e7cdf56e94a0f305dfbc585f4d001
7.感受态细胞的制备.wmv
http://d.namipan.com/d/2053ba246d2f661a17cf52da46a5311c8f857fe8c779d501
8.DNA片段的体外连接.wmv
http://d.namipan.com/d/101d056b43b4e1343912dcfd161246529ccf381eadf6e500
9.探针的标记.wmv
http://d.namipan.com/d/6e3aea7410b7376173895798353d57fa05b50310cd550501
10.探针的检测.wmv
http://d.namipan.com/d/e024fad4e98fcf64a8f877c44aa035d8319a64ed0bfe1801
11.重组DNA的转化.wmv
http://d.namipan.com/d/682ae36c71aa2890bc37ce6dcb3bf8c67d78abf4e70fc702
12.重组子的选择.wmv
http://d.namipan.com/d/b0409400fe56f48ccf832b8bec061a4f1ce5877d45a17b01
13.载体质粒的制备及鉴定.wmv
http://d.namipan.com/d/00887d613029b3be029d95c6977980dfe021decc29d03503
14.目的基因和质粒DNA的限制性内切酶酶切反应.wmv
http://d.namipan.com/d/c256107edcc4ab2d3531e342faa34d298dfb62f8f3193e01
15.外源基因在宿主菌中的诱导表达.wmv
http://d.namipan.com/d/9aa3e5314e8b02c9fca0f6d65669c2c8978a73237df3bb02
16.染色体DNA的酶切与电泳鉴定.wmv
http://d.namipan.com/d/52f224fe9c9d0bb99318c5fa98ebcfc05884eb78c5dd2d01