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Phenomenological Kinetic and Control Analysis of Oxidative Phosphorylation in Isolated Mitochondria

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Metabolic control analysis provides a quantitative framework for analyzing regulatory properties of enzymes in various metabolic pathways. It has been used for estimation of control parameters of the enzymatic pathways at isolated enzyme, cellular, or whole organism levels. This chapter describes how control and elasticities analysis can be experimentally applied to measure control properties of the components of the oxidative phosphorylation system and how a variant of such analysis – phenomenological kinetic analysis – can be used to investigate the effects of various factors (physiological or pathological) on the system of oxidative phosphorylation in isolated mitochondria.
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