Expression of Extracellular N-Terminal Domain of NMDA Receptor in Mammalian Cells
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NMDA receptor is a ligand-gated ion channel receptor composed of multiple subunits encoded by at least five genes and their splice variants (1 ). Functional channels can be produced with only the NR1 subunit. These single-subunit channels, exhibit many of the key properties found in native channels including the direct permeability of calcium, voltage-dependent Mg2+ blockade of the ion channel, and binding sites for modulators, such as Zn2+ , glycine, and polyamines (2 ). The NR1 subunit consists of two functionally distinct regions: a large amino-terminal extracellular domain containing the putative agonist binding site and a hydrophobic core consisting of four transmembrane-spanning domains that participate in the formation of the ion-conducting pathway.
