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Targeting Random Mutations to Hotspots in Antibody Variable Domains for Affinity Improvement

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The potential of Fvs as magic bullets for targeting therapeutic drugs or imaging agents is well-documented. However, the conversion of whole antibodies (Abs) into Fvs (scFvs or dsFvs) is often associated with a drastic reduction in antigen (Ag)-binding affinity. For efficient use of Fvs as targeting agents, this property must be improved.
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