【讨论】有谁正在做RNAa的研究？

请问有谁正在做RNAa的研究，麻烦留个言，我想了解一下国内这方面的研究进展。

在Pubmed里面使用RNAa搜索一下不就什么都搞定了？

# What is RNAa?
RNAa or RNA activation is a novel small RNA-mediated gene regulation mechanism first reported by us [1][2]. RNAa is achieved by targeting carefully selected gene promoter regions using small dsRNAs (21 nt), and such dsRNA molecules are referred to as small activating RNA or saRNA.
# What is saRNA?
saRNA refers to those promoter targeted dsRNAs that can activate sequence-specific gene expression.
# In what organisms has RNAa been observed so far?
RNAa have been demonstrated in different human cells, non-human primate, mouse and rat cell[3], suggesting that RNAa is at least conserved in mammals.
# What are the differences between RNAi and RNAa?
Apparently RNAa is achieved by targeting a gene promoter instead of an mRNA as the classic RNAi is and its end effect is gene activation instead of knockdown. A big difference between these two mechanisms is their kinetics. RNAi effect is know to occur within a couple of hours and disappear 5-7 days following siRNA transfection, while RNAa does not appear until about 48 hrs following transfection and lasts much longer (at least two weeks).
# How to achieve optimal RNAa in in vitro transfection experiments?
First, general guidelines for transfecting dsRNAs into mammalian cells should be followed. We found that this transfection protocol helpful for achieving optimal gene activation. Here are some important tips: 1) Reverse transfection works better than forward transfection (many vendors of transfection reagents provide reverse transfection protocols); 2) Harvest cells for expression analysis at day 4 or day 5 following transfection; 3) At transfection, cell density should be such that at harvest they are not overcrowded.

国内相关文献很少有报道，Pubmed上还是有几篇重要的文献搜不到全文，希望有关人士能支持一些资源。小弟不才，想做一些这方面的研究。以下是不能搜到全文的文章：Novel functions for small RNA molecules.
Defining features and exploring chemical modifications to manipulate RNAa activity
Double strand RNA-guided endogeneous E-cadherin up-regulation induces the apoptosis and inhibits proliferation of breast carcinoma cells in vitro and in vivo.
Double-stranded siRNA targeted to the huntingtin gene does not induce DNA methylation
MicroRNA-373 induces expression of genes with complementary promoter sequences)
Small interfering RNA directed transcriptional activation in human cells
Small Interfering RNA-Induced Transcriptional Gene Silencing in Human Cells
Up-regulation of E-cadherin by small activating RNA inhibits cell invasion and migration in 5637 human bladder cancer cells.
这几篇都是该方面引用相当多的文章。

多谢版主指点

请问楼主做RNAa了吗？做得怎么样？我准备做这方面的，有什么经验可以学习吗？QQ：442895241 Email:slpan7809@yahoo.com.cn