Oligonucleotide Arrays for Genotyping: Enzymatic Methods for Typing Single Nucleotide Polymorphisms and Short Tandem Repeats

Much of modern genetics is based on analysis of DNA sequence. Therefore, there is great pressure to scale up sequence analysis while decreasing its cost. The most promising platforms are based on the use of oligonucleotide arrays (DNA chips), which perform many analyses in parallel (1 ). Arrays comprise libraries of oligonucleotides or polynucleotides attached to solid supports at defined locations. Assays can be performed on the whole library simultaneously, increasing both the speed and accuracy of analysis of the target nucleic acid.