蛋白质组学-质谱分析

Proteomics Primer

1. Proteomics
2. 2-D PAGE
3. Immobilised pH gradients (IPGs)
4. Mass spectrometry
5. Principles of mass spectrometry
6. Matrix assisted laser desorption/ionisation (MALDI)
7. Electrospray ionisation
8. Mass Analysers?- Time-of-flight analysers
9. Mass Analysers - Quadrupole mass analyser???
10. Application of MALDI-TOF in proteomics
11. Protein identification????
12. Study of proteins and peptides by ESI
13. Tandem mass spectrometry
14. Post source decay (PSD)
15. Sequencing of peptides using PSD
16. Tandem mass spectrometry using electrospray ionisation
17. Protein identification using ESI-MS/MS
18. Hybrid quadrupole-TOF (Q-TOF) for MS/MS
19. Protein-Protein interactions
20. Mass spectrometric identification of complexes
21. Alternatives to 2-D PAGE
22. References