【共享】microRNA protocols (english edition)
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MicroRNA Protocols
Edited by Shao-Yao Ying
Department of Cell and Neurobiology, Keck School of Medicine,
University of Southern California, Los Angeles, CA
Contents
Preface ............................................v
Contributors .......................................xi
1. The MicroRNA: Overview of the RNA Gene That Modulates
Gene Functions Shao-Yao Ying, Donald C. Chang, Joseph D. Miller, and Shi-Lung Lin ................................................1
2. Structure Analysis of MicroRNA Precursors Jacek Krol and Wlodzimierz J. Krzyzosiak ......................................... 19
3. MicroRNA Biogenesis: Isolation and Characterization of the Microprocessor Complex Richard I. Gregory, Thimmaiah P. Chendrimada, and Ramin Shiekhatter ........................................ 33
4. Recognition and Cleavage of Primary MicroRNA Transcripts Yan Zeng and Bryan R. Cullen ............................................. 49
5. Mouse Embryonic Stem Cells as a Model Genetic System to Dissect and Exploit the RNA Interference Machinery Stefan A. Muljo and ChryssaKanellopoulou ............................... 57
6. MicroRNAs and Messenger RNA Turnover Julia A. Chekanova and Dmitry A. Belostotsky .................................. 73
7. Prediction of MicroRNA Targets MarcRehmsmeier .......................................... 87
8. Prediction of Human MicroRNA Targets
Bino John, Chris Sander, and Debora S. Marks ............... 101
9. Complications in Mammalian MicroRNA Target Prediction
Neil R. Smalheiser and Vetle I. Torvik ............................................. 115
10. miRBase:The MicroRNA Sequence Database Sam Griffiths Jones .............................................. 129
11. Methodologies for High-Throughput Expression Profiling of MicroRNAs
PazEinat............................................ 139
.........................................
Edited by Shao-Yao Ying
Department of Cell and Neurobiology, Keck School of Medicine,
University of Southern California, Los Angeles, CA
Contents
Preface ............................................v
Contributors .......................................xi
1. The MicroRNA: Overview of the RNA Gene That Modulates
Gene Functions Shao-Yao Ying, Donald C. Chang, Joseph D. Miller, and Shi-Lung Lin ................................................1
2. Structure Analysis of MicroRNA Precursors Jacek Krol and Wlodzimierz J. Krzyzosiak ......................................... 19
3. MicroRNA Biogenesis: Isolation and Characterization of the Microprocessor Complex Richard I. Gregory, Thimmaiah P. Chendrimada, and Ramin Shiekhatter ........................................ 33
4. Recognition and Cleavage of Primary MicroRNA Transcripts Yan Zeng and Bryan R. Cullen ............................................. 49
5. Mouse Embryonic Stem Cells as a Model Genetic System to Dissect and Exploit the RNA Interference Machinery Stefan A. Muljo and ChryssaKanellopoulou ............................... 57
6. MicroRNAs and Messenger RNA Turnover Julia A. Chekanova and Dmitry A. Belostotsky .................................. 73
7. Prediction of MicroRNA Targets MarcRehmsmeier .......................................... 87
8. Prediction of Human MicroRNA Targets
Bino John, Chris Sander, and Debora S. Marks ............... 101
9. Complications in Mammalian MicroRNA Target Prediction
Neil R. Smalheiser and Vetle I. Torvik ............................................. 115
10. miRBase:The MicroRNA Sequence Database Sam Griffiths Jones .............................................. 129
11. Methodologies for High-Throughput Expression Profiling of MicroRNAs
PazEinat............................................ 139
.........................................
