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Solexa Primer/Adapter Sequences

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Sequences for Solexa Library Preparations:

Genomic DNA oligonucleotide sequences

Adapters 1
  5' P-GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG
  5' ACACTCTTTCCCTACACGACGCTCTTCCGATCT

PCR Primers 1
  5' AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT TCCGATCT
  5' CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT

Genomic DNA Sequencing Primer
  5' ACACTCTTTCCCTACACGACGCTCTTCCGATCT

DpnII gene expression oligonucleotide sequences

Gex Adapter 1
  5' P-GATCGTCGGACTGTAGAACTCTGAAC
  5'ACAGGTTCAGAGTTCTACAGTCCGAC

Gex Adapter 2
  5' CAAGCAGAAGACGGCATACGANN
  5' P-TCGTATGCCGTCTTCTGCTTG

Gex PCR Primer 1
  5' CAAGCAGAAGACGGCATACGA

Gex PCR Primer 2
  5' AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA

Gex Sequencing Primer
  5' CGACAGGTTCAGAGTTCTACAGTCCGACGATC

NlaIII gene expression oligonucleotide sequences

Gex Adapter 1
  5' P-TCGGACTGTAGAACTCTGAAC
  5' ACAGGTTCAGAGTTCTACAGTCCGACATG

Gex Adapter 2
  5' CAAGCAGAAGACGGCATACGANN
  5' P-TCGTATGCCGTCTTCTGCTTG

Gex PCR Primer 1
  5' CAAGCAGAAGACGGCATACGA

Gex PCR Primer 2
  5' AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA

Gex Sequencing Primer
  5' CCGACAGGTTCAGAGTTCTACAGTCCGACATG

Small RNA oligonucleotide sequences

RT Primer
  5' CAAGCAGAAGACGGCATACGA

5' RNA Adapter
  5' GUUCAGAGUUCUACAGUCCGACGAUC

3' RNA Adapter
  5' P-UCGUAUGCCGUCUUCUGCUUGUidT

Small RNA PCR Primer 1
  5' CAAGCAGAAGACGGCATACGA

Small RNA PCR Primer 2
  5' AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA

Small RNA Sequencing Primer
  5' CGACAGGTTCAGAGTTCTACAGTCCGACGATC

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