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2%多聚甲醛固定剂配方二则

互联网

3314

METHOD 1:

Formaldehyde preservative 2% formaldehyde solution in protein-free
phosphate-buffered saline (PBS).

Prepare as follows:

Add 2 g paraformaldehyde powder (e.g., Sigma, St. Louis, MO) to 100 ml
of 1 X PBS. Heat to 70
° C (do not exceed this temperature) in a fume hood
until the paraformaldehyde goes into solution (note that this happens
quickly as soon as the suspension reaches 70
° C). Allow the solution to
cool to room temperature. Adjust to pH 7.4 using 0.1 M NaOH or 0.1 M HCl,
if needed. Filter and store at 4
° C protected from light.

METHOD 2:

Formaldehyde preservative 2% formaldehyde solution in protein-free

PBS.

Prepare as follows:

10% formaldehydundefined    20 ml
10 x PBS
       10 ml
Distilled water
     70 ml

Cat.#04018), depolymerized paraformaldehyde, EM grade, methanol-free solution.

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