Thermal Cycle Dideoxy DNA Sequencing

Since DNA sequencing has rapidly become standard practice in many laboratories, a large variety of new cloning vectors, sequencing strategies, and techniques have been developed to allow more efficient sequencing of a large variety of DNA templates. Despite a current focus on the automation of DNA sequencing procedures, other methods are still required until automated DNA sequencing is in more general use. One recent addition to this repertoire of sequencing methods is termed thermal cycle sequencing.