【旧贴整理】半定量PCR
丁香园论坛
9213
1.选择方法（同管或是不同管）
http://www.dxy.cn/bbs/thread/8043289
2.循环次数选择
http://www.dxy.cn/bbs/actions/archive/post/1701400_0.html
http://www.dxy.cn/bbs/actions/archive/post/4882246_0.html
3.内参比目的片断亮
http://www.dxy.cn/bbs/actions/archive/post/4542410_0.html
4.半定量PCR原理
http://www.dxy.cn/bbs/actions/archive/post/3954294_0.html
http://www.dxy.cn/bbs/actions/archive/post/3953811_0.html
http://www.dxy.cn/bbs/post/view?bid=67&id=3954294&sty=3&keywords=%B0%EB%B6%A8%C1%BFPCR
5.软件分析
http://www.dxy.cn/bbs/actions/archive/post/2302644_0.html
6.步骤
http://www.dxy.cn/bbs/actions/archive/post/4874590_0.html
http://www.dxy.cn/bbs/thread/9036424
7.定量pcr、半定量pcr、相对定量pcr差异
http://www.dxy.cn/bbs/thread/7960801
8.方法
http://www.dxy.cn/bbs/actions/archive/post/231443_0.html
9.排除基因组DNA的干扰
http://www.dxy.cn/bbs/actions/archive/post/4492454_0.html
10。重复性
http://www.dxy.cn/bbs/actions/archive/post/5059190_0.html
11.分管扩增具体方法
http://www.dxy.cn/bbs/post/view?bid=67&id=289521&sty=3&keywords=%B0%EB%B6%A8%C1%BFPCR
http://www.dxy.cn/bbs/actions/archive/post/7573648_0.html
12.荧光半定量PCR分析
http://www.dxy.cn/bbs/actions/archive/post/113108_0.html
13.精华贴
http://www.dxy.cn/bbs/post/view?bid=67&id=708668&sty=3&keywords=%B0%EB%B6%A8%C1%BFPCR
14.内标
http://www.dxy.cn/bbs/actions/archive/post/1048050_0.html
15.RNA抽提与半定量PCR
http://www.dxy.cn/bbs/actions/archive/post/3761937_0.html
16.概念
http://www.dxy.cn/bbs/actions/archive/post/4250899_0.html
http://www.dxy.cn/bbs/post/view?bid=67&id=2040253&sty=3&keywords=%B0%EB%B6%A8%C1%BFPCR
17。细菌半定量PCR内参
http://www.dxy.cn/bbs/thread/9280499
18.内参和目的基因表达与循环次数的关系
http://www.dxy.cn/bbs/actions/archive/post/5764465_0.html
http://www.dxy.cn/bbs/actions/archive/post/5772365_0.html
19。是否需要测序
http://www.dxy.cn/bbs/actions/archive/post/2841144_0.html
20。内参的扩增
http://www.dxy.cn/bbs/post/view?bid=67&id=4402061&sty=3&keywords=%B0%EB%B6%A8%C1%BFPCR
21。分析方法
http://www.dxy.cn/bbs/actions/archive/post/2788600_0.html
22.时新性
http://www.dxy.cn/bbs/post/view?bid=64&id=7815625&sty=3&keywords=%B0%EB%B6%A8%C1%BFPCR
23.杂带的处理方法
http://www.dxy.cn/bbs/actions/archive/post/5115043_0.html
http://www.dxy.cn/bbs/thread/8043289
2.循环次数选择
http://www.dxy.cn/bbs/actions/archive/post/1701400_0.html
http://www.dxy.cn/bbs/actions/archive/post/4882246_0.html
3.内参比目的片断亮
http://www.dxy.cn/bbs/actions/archive/post/4542410_0.html
4.半定量PCR原理
http://www.dxy.cn/bbs/actions/archive/post/3954294_0.html
http://www.dxy.cn/bbs/actions/archive/post/3953811_0.html
http://www.dxy.cn/bbs/post/view?bid=67&id=3954294&sty=3&keywords=%B0%EB%B6%A8%C1%BFPCR
5.软件分析
http://www.dxy.cn/bbs/actions/archive/post/2302644_0.html
6.步骤
http://www.dxy.cn/bbs/actions/archive/post/4874590_0.html
http://www.dxy.cn/bbs/thread/9036424
7.定量pcr、半定量pcr、相对定量pcr差异
http://www.dxy.cn/bbs/thread/7960801
8.方法
http://www.dxy.cn/bbs/actions/archive/post/231443_0.html
9.排除基因组DNA的干扰
http://www.dxy.cn/bbs/actions/archive/post/4492454_0.html
10。重复性
http://www.dxy.cn/bbs/actions/archive/post/5059190_0.html
11.分管扩增具体方法
http://www.dxy.cn/bbs/post/view?bid=67&id=289521&sty=3&keywords=%B0%EB%B6%A8%C1%BFPCR
http://www.dxy.cn/bbs/actions/archive/post/7573648_0.html
12.荧光半定量PCR分析
http://www.dxy.cn/bbs/actions/archive/post/113108_0.html
13.精华贴
http://www.dxy.cn/bbs/post/view?bid=67&id=708668&sty=3&keywords=%B0%EB%B6%A8%C1%BFPCR
14.内标
http://www.dxy.cn/bbs/actions/archive/post/1048050_0.html
15.RNA抽提与半定量PCR
http://www.dxy.cn/bbs/actions/archive/post/3761937_0.html
16.概念
http://www.dxy.cn/bbs/actions/archive/post/4250899_0.html
http://www.dxy.cn/bbs/post/view?bid=67&id=2040253&sty=3&keywords=%B0%EB%B6%A8%C1%BFPCR
17。细菌半定量PCR内参
http://www.dxy.cn/bbs/thread/9280499
18.内参和目的基因表达与循环次数的关系
http://www.dxy.cn/bbs/actions/archive/post/5764465_0.html
http://www.dxy.cn/bbs/actions/archive/post/5772365_0.html
19。是否需要测序
http://www.dxy.cn/bbs/actions/archive/post/2841144_0.html
20。内参的扩增
http://www.dxy.cn/bbs/post/view?bid=67&id=4402061&sty=3&keywords=%B0%EB%B6%A8%C1%BFPCR
21。分析方法
http://www.dxy.cn/bbs/actions/archive/post/2788600_0.html
22.时新性
http://www.dxy.cn/bbs/post/view?bid=64&id=7815625&sty=3&keywords=%B0%EB%B6%A8%C1%BFPCR
23.杂带的处理方法
http://www.dxy.cn/bbs/actions/archive/post/5115043_0.html
