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【旧贴整理】荧光定量PCR整理贴

丁香园论坛

1071
1.原理,基本步骤
http://www.dxy.cn/bbs/post/view?bid=67&id=4476935&sty=3&keywords=%D3%AB%B9%E2%B6%A8%C1%BFPCR
http://www.dxy.cn/bbs/post/view?bid=67&id=9466944&sty=3&keywords=%D3%AB%B9%E2%B6%A8%C1%BFPCR
http://www.dxy.cn/bbs/post/view?bid=67&id=8301747&sty=3&keywords=%D3%AB%B9%E2%B6%A8%C1%BFPCR
http://www.dxy.cn/bbs/actions/archive/post/4325861_0.html
http://www.dxy.cn/bbs/actions/archive/post/5350286_0.html
http://www.dxy.cn/bbs/post/view?bid=38&id=30872&sty=3&keywords=%D3%AB%B9%E2%B6%A8%C1%BFPCR
http://www.dxy.cn/bbs/post/view?bid=67&id=7170159&sty=3&keywords=%D3%AB%B9%E2%B6%A8%C1%BFPCR
http://www.dxy.cn/bbs/post/view?bid=154&id=5019416&sty=3&keywords=%D3%AB%B9%E2%B6%A8%C1%BFPCR
http://www.dxy.cn/bbs/post/view?bid=67&id=11960851&sty=3&keywords=%D3%AB%B9%E2%B6%A8%C1%BFPCR

2.价格
http://www.dxy.cn/bbs/actions/archive/post/282001_0.html
http://www.dxy.cn/bbs/post/view?bid=67&id=236417&sty=3&keywords=%D3%AB%B9%E2%B6%A8%C1%BFPCR
http://www.dxy.cn/bbs/actions/archive/post/7814577_0.html
http://www.dxy.cn/bbs/post/view?bid=67&id=2222228&sty=3&keywords=%D3%AB%B9%E2%B6%A8%C1%BFPCR
http://www.dxy.cn/bbs/thread/9778865

3.仪器购买
http://www.dxy.cn/bbs/actions/archive/post/4222783_0.html
http://www.dxy.cn/bbs/post/view?bid=67&id=3600471&sty=3&keywords=%D3%AB%B9%E2%B6%A8%C1%BFPCR
http://www.dxy.cn/bbs/actions/archive/post/4219747_0.html

4,结果分析:
无CT值
http://www.dxy.cn/bbs/thread/10640243
荧光定量PCR方法中△△Ct法
http://www.dxy.cn/bbs/post/view?bid=154&id=12016170&sty=3&keywords=%D3%AB%B9%E2%B6%A8%C1%BFPCR
http://www.dxy.cn/bbs/actions/archive/post/8150485_0.html
http://www.dxy.cn/bbs/thread/11955176
染料法荧光定量PCR实例求教
http://www.dxy.cn/bbs/post/view?bid=67&id=10868447&sty=3&keywords=%D3%AB%B9%E2%B6%A8%C1%BFPCR

5.技术专辑
http://www.dxy.cn/bbs/post/view?bid=67&id=9646584&sty=3&keywords=%D3%AB%B9%E2%B6%A8%C1%BFPCR
SYBR染料标记荧光定量PCR
http://www.dxy.cn/bbs/actions/archive/post/5420190_0.html
荧光定量PCR的试剂
荧光定量PCR的退火温度
http://www.dxy.cn/bbs/thread/12117358
MMP2荧光定量PCR引物设计
http://www.dxy.cn/bbs/thread/9350078
荧光定量PCR如何设计引物
http://www.dxy.cn/bbs/actions/archive/post/292345_0.html
http://www.dxy.cn/bbs/actions/archive/post/2974589_0.html
http://www.dxy.cn/bbs/post/view?bid=67&id=11196972&sty=3&keywords=%D3%AB%B9%E2%B6%A8%C1%BFPCR
荧光定量PCR技术要领
http://www.dxy.cn/bbs/post/view?bid=67&id=8203547&sty=3&keywords=%D3%AB%B9%E2%B6%A8%C1%BFPCR
荧光定量PCR的模板问题
http://www.dxy.cn/bbs/thread/10957097
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