N-Terminal Amino Acid Sequencing of 2-DE Spots

Two-dimensional electrophoresis (2-DE) is one of the most advanced techniques for direct separation of considerable numbers of proteins. Direct microsequence analysis of the amino (N)-terminal partial sequence of the electroblotted protein spots from the 2-DE gels and the homology search against protein sequence database(s) have provided identification of proteins in addition to the conventional identification processes, such as comigration.