Silane and Poly-L-Lysine Coating of Microscope Slides

 

Silane and Poly-L-Lysine Coating of Microscope Slides

1) Wash slides in Decon solution for 30 minutes.

2) Wash slides in running tap water for 30 minutes.

3) Wash slides in distilled water for 2 x 5 minutes.

4) Wash slides in 95% ethanol for 2 x 5 minutes.

5) Air dry in a dust free environment for 5 minutes.

Poly-L-Lysine Coating

a) Smear 20ul of poly-l-lysine over each slide.
b) Air dry in a dust free environment.

Silated Slides

a) To 300mls acetone add 6mls Aminopropyltriethoxysilane solution (Sigma A-3648).
b) Dip slides into silane solution for 10 seconds.
c) Wash 2 x briefly in distilled water.
d) Dry overnight at 37°C in a dust-free environment.