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Silane and Poly-L-Lysine Coating of Microscope Slides

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954

 

1) Wash slides in Decon solution for 30 minutes.

2) Wash slides in running tap water for 30 minutes.

3) Wash slides in distilled water for 2 x 5 minutes.

4) Wash slides in 95% ethanol for 2 x 5 minutes.

5) Air dry in a dust free environment for 5 minutes.

Poly-L-Lysine Coating

    a) Smear 20ul of poly-l-lysine over each slide.
    b) Air dry in a dust free environment.

Silated Slides

    a) To 300mls acetone add 6mls Aminopropyltriethoxysilane solution (Sigma A-3648).
    b) Dip slides into silane solution for 10 seconds.
    c) Wash 2 x briefly in distilled water.
    d) Dry overnight at 37°C in a dust-free environment.

 

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