Preparation of Mammalian Oocytes for Transmission Electron Microscopy
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The visualization of subcellular organelles and structures is a valuable tool for understanding cellular changes that occur
in oocytes and early embryos as a result of genetic alterations, incubation conditions, drug treatments, and many other manipulations.
Preparing oocytes for transmission electron microscopic analysis can be challenging as these cells cannot be visualized without
a microscope and they are more susceptible to mechanical disruption during manipulation. Here we describe methods for immobilizing
oocytes on either a solid surface or within a matrix and then document our embedding techniques which work well for preserving
the ultrastructure of the mouse oocyte.
