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Use of Transmission Electron Microscopy

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  Use of Transmission Electron Microscopy  
 
  Overview
  A protocol describing the use of Zeiss EM9-S transmission electron microscopy is presented. 
 
  Material
  Zeiss EM9-S transmission electron microscope  
 
  Procedure
  1. Familiarize yourself with the basic operations of Fixation, embedding and sectioning of materials for viewing in an electron microscope.

2. Insert and Remove a Specimen from the chamber.

3. Turn on the high voltage, check current levels and turn on the beam.

4. Find and center the beam.

5. Use magnification controls and condenser.

6. Use binocular viewing screen

7. Take an electron photomicrograph.

 

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