EPON resin mixture for transmission electron microscopy

 

EPON resin mixture for transmission electron microscopy

For Epon WPE 153:

			~120 ml	~60 ml	~30 ml
Mix A:	Embed 812	44 ml	22.1 ml	11.1 ml
	DDSA	67 ml	33.3 ml	16.7 ml
Mix B:		Embed 812	67 ml	33.3 ml	16.7 ml
	NMA	56 ml	27.8 ml	13.9 ml

Prepare mixture A and mixture B in 30 - 120 ml batches. Mix well and store in glass scintillation vials or some other container that can be capped tightly. Store at -20 degrees C. The mixtures can be stored for years.

For use:

1. Be certain to completely warm the vials before opening them to prevent water from mixing with the plastic.
2. Mix equal volumes of A and B together.
3. Then add 0.1 ml of DMP-30 for each 5 ml of plastic.
4. Mix well and store no longer than a day at room temp. I usually put the remainder of the plastic in a tube or vial, cap and wrap the cap with parafilm, and put it at 4 degrees C overnight.