DiOlistics: Delivery of Fluorescent Dyes into Cells

DiOlistic labeling utilizes a particle-mediated delivery system to incorporate dye into cells. Because of its random nature, this technique generates sparse fluorescent labeling which is well suited for the study of neuronal dendritic branching and dendritic spine morphology. DiOlistics is a quick, reliable and nontoxic method that can be used in combination with other techniques such as immunostaining, biolistic DNA transfection, and retrograde tracing. In this article, we describe the methods for diOlistic labeling of neurons from rodent brain slices using DiI and the imaging of neuronal and synaptic morphology using confocal microscopy.