寻找原核表达载体
丁香园论坛
1302
请问有哪些原核表达载体的mcs含有以下三个以上以下的酶切位点。谢谢
AarI CACCTGC,4,8 MfeI C/AATTG
AatII GACGT/C MluI A/CGCGT
AccI GT/MKAC MmeI TCCRAC,20,18
AceII GCTAG/C MscI TGG/CCA
AclI AA/CGTT NaeI GCC/GGC
AfeI AGC/GCT NarI GG/CGCC
AflII C/TTAAG NdeI CA/TATG
AgeI A/CCGGT NgoMIV G/CCGGC
AhdI Gundefined*N~HNNGTC_NheI_G~HCTAGC~Kbr_~H~M~2~1ApaI_GGGCC~HC_NotI_GC~HGGCCGC~Kbr_~H~M~2~1ApaLI_G~HTGCAC_NruI_TCG~HCGA~Kbr_~H~M~2~1AscI_GG~HCGCGCC_NsiI_ATGCA~HT~Kbr_~H~M~2~1AseI_AT~HTAAT_OliI_Cundefined*~HNNGTG~Kbr_~H~M~2~1AsiSI_GCGAT~HCGC_PacI_TTAAT~HTAA~Kbr_~H~M~2~1AvrII_C~HCTAGG_PciI_A~HCATGT~Kbr_~H~M~2~1BaeI_undefined*NNGTAYC~E12~E7_PmeI_GTTT~HAAAC~Kbr_~H~M~2~1BbeI_GGCGC~HC_PmlI_CAC~HGTG~Kbr_~H~M~2~1BbvCI_CCTCAGC~E~F5~E~F2_PpiI_GAundefined*NNNCTC~E13~E8~Kbr_~H~M~2~1BclI_T~HGATCA_Ppu10I_A~HTGCAT~Kbr_~H~M~2~1BfrBI_ATG~HCAT_PpuMI_RG~HGWCCY~Kbr_~H~M~2~1BglI_Gundefined*NN~HNGGC_PshAI_Gundefined*~HNNGTC~Kbr_~H~M~2~1Bpu10I_CCTNAGC~E~F5~E~F2_PsiI_TTA~HTAA~Kbr_~H~M~2~1BsaAI_YAC~HGTR_PspOMI_G~HGGCCC~Kbr_~H~M~2~1BsaHI_GR~HCGYC_PsrI_GAundefined*NNNNTA~HC~Kbr_~H~M~2~1BsgI_GTGCAG~E16~E14_PvuI_CGAT~HCG~Kbr_~H~M~2~1BsiEI_CGRY~HCG_RleAI_CCCACA~E12~E9~Kbr_~H~M~2~1BsiWI_C~HGTACG_RsrII_CG~HGWCCG~Kbr_~H~M~2~1BsmBI_C~HGTCTC_SacII_CCGC~HGG~Kbr_~H~M~2~1BsrBI_CCGCTC~E~F3~E~F3_SalI_G~HTCGAC~Kbr_~H~M~2~1BsrFI_R~HCCGGY_SanDI_GG~HGWCCC~Kbr_~H~M~2~1BsrGI_T~HGTACA_SapI_G~HCTCTTC~Kbr_~H~M~2~1BssHII_G~HCGCGC_SbfI_CCTGCA~HGG~Kbr_~H~M~2~1BssSI_CACGAG~E~F5~E~F1_ScaI_AGT~HACT~Kbr_~H~M~2~1BstBI_TT~HCGAA_SciI_CTC~HGAG~Kbr_~H~M~2~1BstEII_G~HGTNACC_SelI_~HCGCG~Kbr_~H~M~2~1BstUI_CG~HCG_SexAI_A~HCCWGGT~Kbr_~H~M~2~1BstXI_CCANNNNN~HNTGG_SfiI_GGundefined*NN~HNGGCC~Kbr_~H~M~2~1BstZ17I_GTA~HTAC_SfoI_GGC~HGCC~Kbr_~H~M~2~1Bsu36I_CC~HTNAGG_SgrAI_CR~HCCGGYG~Kbr_~H~M~2~1ClaI_AT~HCGAT_SnaBI_TAC~HGTA~Kbr_~H~M~2~1DraI_TTT~HAAA_SpeI_A~HCTAGT~Kbr_~H~M~2~1DrdI_Gundefined*NN~HNNGTC_SphI_GCATG~HC~Kbr_~H~M~2~1EaeI_Y~HGGCCR_SrfI_GCCC~HGGGC~Kbr_~H~M~2~1EagI_C~HGGCCG_Sse232I_CG~HCCGGCG~Kbr_~H~M~2~1EciI_GGCGGA~E11~E9_Sse8647I_AG~HGWCCT~Kbr_~H~M~2~1FauI_CCCGC~E4~E6_SwaI_ATTT~HAAAT~Kbr_~H~M~2~1FseI_GGCCGG~HCC_TaqII_GACCGA~E11~E9~Kbr_~H~M~2~1FspAI_RTGC~HGCAY_TauI_GCSG~HC~Kbr_~H~M~2~1FspI_TGC~HGCA_Tth111I_GACN~HNNGTC~Kbr_~H~M~2~1GdiII_CGGCCR~E~F5~E~F1_XbaI_T~HCTAGA~Kbr_~H~M~2~1HgaI_GACGC~E5~E10_XhoI_C~HTCGAG~Kbr_~H~M~2~1HpaI_GTT~HAAC_XmnI_GAANN~HNNTTC~Kbr_~H~M~2~1KasI_G~HGCGCC_ZraI_GAC~HGTC
AarI CACCTGC,4,8 MfeI C/AATTG
AatII GACGT/C MluI A/CGCGT
AccI GT/MKAC MmeI TCCRAC,20,18
AceII GCTAG/C MscI TGG/CCA
AclI AA/CGTT NaeI GCC/GGC
AfeI AGC/GCT NarI GG/CGCC
AflII C/TTAAG NdeI CA/TATG
AgeI A/CCGGT NgoMIV G/CCGGC
AhdI Gundefined*N~HNNGTC_NheI_G~HCTAGC~Kbr_~H~M~2~1ApaI_GGGCC~HC_NotI_GC~HGGCCGC~Kbr_~H~M~2~1ApaLI_G~HTGCAC_NruI_TCG~HCGA~Kbr_~H~M~2~1AscI_GG~HCGCGCC_NsiI_ATGCA~HT~Kbr_~H~M~2~1AseI_AT~HTAAT_OliI_Cundefined*~HNNGTG~Kbr_~H~M~2~1AsiSI_GCGAT~HCGC_PacI_TTAAT~HTAA~Kbr_~H~M~2~1AvrII_C~HCTAGG_PciI_A~HCATGT~Kbr_~H~M~2~1BaeI_undefined*NNGTAYC~E12~E7_PmeI_GTTT~HAAAC~Kbr_~H~M~2~1BbeI_GGCGC~HC_PmlI_CAC~HGTG~Kbr_~H~M~2~1BbvCI_CCTCAGC~E~F5~E~F2_PpiI_GAundefined*NNNCTC~E13~E8~Kbr_~H~M~2~1BclI_T~HGATCA_Ppu10I_A~HTGCAT~Kbr_~H~M~2~1BfrBI_ATG~HCAT_PpuMI_RG~HGWCCY~Kbr_~H~M~2~1BglI_Gundefined*NN~HNGGC_PshAI_Gundefined*~HNNGTC~Kbr_~H~M~2~1Bpu10I_CCTNAGC~E~F5~E~F2_PsiI_TTA~HTAA~Kbr_~H~M~2~1BsaAI_YAC~HGTR_PspOMI_G~HGGCCC~Kbr_~H~M~2~1BsaHI_GR~HCGYC_PsrI_GAundefined*NNNNTA~HC~Kbr_~H~M~2~1BsgI_GTGCAG~E16~E14_PvuI_CGAT~HCG~Kbr_~H~M~2~1BsiEI_CGRY~HCG_RleAI_CCCACA~E12~E9~Kbr_~H~M~2~1BsiWI_C~HGTACG_RsrII_CG~HGWCCG~Kbr_~H~M~2~1BsmBI_C~HGTCTC_SacII_CCGC~HGG~Kbr_~H~M~2~1BsrBI_CCGCTC~E~F3~E~F3_SalI_G~HTCGAC~Kbr_~H~M~2~1BsrFI_R~HCCGGY_SanDI_GG~HGWCCC~Kbr_~H~M~2~1BsrGI_T~HGTACA_SapI_G~HCTCTTC~Kbr_~H~M~2~1BssHII_G~HCGCGC_SbfI_CCTGCA~HGG~Kbr_~H~M~2~1BssSI_CACGAG~E~F5~E~F1_ScaI_AGT~HACT~Kbr_~H~M~2~1BstBI_TT~HCGAA_SciI_CTC~HGAG~Kbr_~H~M~2~1BstEII_G~HGTNACC_SelI_~HCGCG~Kbr_~H~M~2~1BstUI_CG~HCG_SexAI_A~HCCWGGT~Kbr_~H~M~2~1BstXI_CCANNNNN~HNTGG_SfiI_GGundefined*NN~HNGGCC~Kbr_~H~M~2~1BstZ17I_GTA~HTAC_SfoI_GGC~HGCC~Kbr_~H~M~2~1Bsu36I_CC~HTNAGG_SgrAI_CR~HCCGGYG~Kbr_~H~M~2~1ClaI_AT~HCGAT_SnaBI_TAC~HGTA~Kbr_~H~M~2~1DraI_TTT~HAAA_SpeI_A~HCTAGT~Kbr_~H~M~2~1DrdI_Gundefined*NN~HNNGTC_SphI_GCATG~HC~Kbr_~H~M~2~1EaeI_Y~HGGCCR_SrfI_GCCC~HGGGC~Kbr_~H~M~2~1EagI_C~HGGCCG_Sse232I_CG~HCCGGCG~Kbr_~H~M~2~1EciI_GGCGGA~E11~E9_Sse8647I_AG~HGWCCT~Kbr_~H~M~2~1FauI_CCCGC~E4~E6_SwaI_ATTT~HAAAT~Kbr_~H~M~2~1FseI_GGCCGG~HCC_TaqII_GACCGA~E11~E9~Kbr_~H~M~2~1FspAI_RTGC~HGCAY_TauI_GCSG~HC~Kbr_~H~M~2~1FspI_TGC~HGCA_Tth111I_GACN~HNNGTC~Kbr_~H~M~2~1GdiII_CGGCCR~E~F5~E~F1_XbaI_T~HCTAGA~Kbr_~H~M~2~1HgaI_GACGC~E5~E10_XhoI_C~HTCGAG~Kbr_~H~M~2~1HpaI_GTT~HAAC_XmnI_GAANN~HNNTTC~Kbr_~H~M~2~1KasI_G~HGCGCC_ZraI_GAC~HGTC
