【分享】电子书《PCR Primer Design》

Book Description

In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes. New high-throughput methods have been developed for genome sequencing and studying the cell at different systematic levels such as transcriptome, proteome, metabolome and other -omes. At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). PCR Primer Design focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, this volume is an exceptional reference for all those involved in studying the genome.

In PCR Primer Design, authors describe basic approaches for PCR primer design in addition to specialized methods. These state-of-the-art methods can be used for both genome-scale experiments and for small-scale individual PCR amplifications. This volume will be useful for organizations performing whole genome studies, companies designing instruments that utilize PCR, and individual scientists geneticists, molecular biologists, molecular geneticists, and more who routinely use PCR in their research.

1. Physical Principles and Visual-OMP Software for Optimal PCR Design
John SantaLucia
pp. 3 - 34


2. OLIGO 7 Primer Analysis Software
Wojciech Rychlik
pp. 35 - 60


3. Selection for 3′-End Triplets for Polymerase Chain Reaction Primers
Kenji Onodera
pp. 61 - 74


4. The Reference Point Method in Primer Design
Thomas Kämpke
pp. 75 - 92


5. PCR Primer Design Using Statistical Modeling
Anton Yuryev
pp. 93 - 104


6. Developing a Statistical Model for Primer Design
Jianping Huang; Anton Yuryev
pp. 105 - 138


7. GST-PRIME: An Algorithm for Genome-Wide Primer Design
Dario Leister; Claudio Varotto
pp. 141 - 158


8. Genome-Scale Probe and Primer Design with PRIMEGENS
Gyan Prakash Srivastava; Dong Xu
pp. 159 - 176


9. SNPbox: Web-Based High-Throughput Primer Design with an Eye for Repetitive Sequences
Stefan Weckx; Peter De Rijk; Wim Glassee; Christine Van Broeckhoven; Jurgen Del-Favero
pp. 179 - 200


10. Fast Masking of Repeated Primer Binding Sites in Eukaryotic Genomes
Reidar Andreson; Lauris Kaplinski; Maido Remm
pp. 201 - 218

11. Degenerate Primer Design: Theoretical Analysis and the HYDEN Program
Chaim Linhart; Ron Shamir
pp. 221 - 244


12. An Iterative Method for Selecting Degenerate Multiplex PCR Primers
Richard Souvenir; Jeremy Buhler; Gary Stormo; Weixiong Zhang
pp. 245 - 268


13. Primer Design for Multiplexed Genotyping
Lars Kaderali
pp. 269 - 286


14. MultiPLX: Automatic Grouping and Evaluation of PCR Primers
Lauris Kaplinski; Maido Remm
pp. 287 - 304


15. MultiPrimer: A System for Microarray PCR Primer Design
Rohan Fernandes; Steven Skiena
pp. 305 - 314


16. Modified Oligonucleotides as Tools for Allele-Specific Amplification
Michael Strerath; Ilka Detmer; Jens Gaster; Andreas Marx
pp. 317 - 328


17. AlleleID: A Pathogen Detection and Identification System
Arun Apte; Siddharth Singh
pp. 329 - 346


18. Designing Primers for Whole Genome PCR Scanning Using the Software Package GenoFrag: A Software Package for the Design of Primers Dedicated to Whole-Genome Scanning by LR-PCR
Nouri Ben Zakour; Yves Le Loir
pp. 349 - 368


19. Designing PCR Primer for DNA Methylation Mapping
Long-Cheng Li
pp. 371 - 384


20. BiSearch: ePCR Tool for Native or Bisulfite-Treated Genomic Template
Tamás Arányi; Gábor E. Tusnády
pp. 385 - 402

21. Graphical Design of Primers with PerlPrimer
Owen Marshall
pp. 403 - 414

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