第一次设计引物，请大家给评价一下!多谢！！
丁香园论坛
932
上游引物 5’－GGAA CCAGTTCGTC CCCAGCCAA -3’
下游引物 5-CACCAAAGTCCCAGGAGGCCG-3
1 cggcctcaac aacgtccctt actccgccaa gagcagctat gacctgggct acaccgccgc
61 ctacacctcc tacgctccct atggaaccag ttcgtcccca gccaacaacg agcctgagaa
121 ggaggacctt gagcctgaaa ttcggatagt gaacgggaag ccaaagaaag tccggaaacc
181 ccgcaccatc tactccagtt tccagctggc ggctcttcag cggcgtttcc aaaagactca
241 gtacttggcc ttgccggagc gagccgagct ggcggcctct ctgggcctca cccagactca
301 ggtcaaaatc tggttccaga accgccggtc caagttcaag aagatgtgga aaagtggtga
361 gatcccctcg gagcagcacc ctggggccag cgcttctcca ccttgtgctt cgccgccagt
421 ctcagcgccg gcctcctggg actttggtgt gccgcagcgg atggcgggcg gcggtggtcc
481 gggcagtggc ggcagcggcg ccggcagctc gggctccagc ccgagcagcg cggcctcggc
541 ttttctgggc aactacccct ggtaccacca gacctcggga tccgcctcac acctgcaggc
601 cacggcgccg ctgctgcacc ccactcagac cccgcagccg catcaccacc accaccatca
661 cggcggcggg ggcgccccgg tgagcgcggg gacgattttc taa
1 atgactggag tctttgacag tctagtggct gatatgcact cgacccagat cgccgcctcc
61 agcacgtacc accagcacca gcagcccccg agcggcggcg gcgccggccc gggtggcaac
121 agcagcagca gcagcagcct ccacaagccc caggagtcgc ccacccttcc ggtgtccacc
181 gccaccgaca gcagctacta caccaaccag cagcacccgg cgggcggcgg cggcggcggg
241 ggctcgccct acgcgcacat gggttcctac cagtaccaag ccagcggcct caacaacgtc
301 ccttactccg ccaagagcag ctatgacctg ggctacaccg ccgcctacac ctcctacgct
361 ccctatggaa ccagttcgtc cccagccaac aacgagcctg agaaggagga ccttgagcct
421 gaaattcgga tagtgaacgg gaagccaaag aaagtccgga aaccccgcac catctactcc
481 agtttccagc tggcggctct tcagcggcgt ttccaaaaga ctcagtactt ggccttgccg
541 gagcgagccg agctggcggc ctctctgggc ctcacccaga ctcaggtcaa aatctggttc
601 cagaaccgcc ggtccaagtt caagaagatg tggaaaagtg gtgagatccc ctcggagcag
661 caccctgggg ccagcgcttc tccaccttgt gcttcgccgc cagtctcagc gccggcctcc
721 tgggactttg gtgtgccgca gcggatggcg ggcggcggtg gtccgggcag tggcggcagc
781 ggcgccggca gctcgggctc cagcccgagc agcgcggcct cggcttttct gggcaactac
841 ccctggtacc accagacctc gggatccgcc tcacacctgc aggccacggc gccgctgctg
901 caccccactc agaccccgca gccgcatcac caccaccacc atcacggcgg cgggggcgcc
961 ccggtgagcg cggggacgat tttctaaccc cagggagaac tcgccagaga ctgagagcag
1021 agaccactta tcctcattgc ttaccccgag ccggggttcc ctcctcccgg cccgctgccg
1081 ccacccacct ctcctgcagg ctgcgacctg cagtggcccg tctcaggccc tgctcactcc
1141 cggggccacc aaacgggccc ctctctcggg ggaaccggac agcagcttgg caaaggcctc
1201 cctaaaaggc cgcatttctg acctgagccc cgggtctcgg ctgtttcgag ccccgcctcg
1261 gacttgcctt ccctcccctc cgggtgagcc tgtctggcgc cttcctcgcc ccgggctgag
1321 agctgggtcc cgggagatgg aagcctccca ggcgcgcgag gcttcccggg cgctctgagg
1381 cttctttctc ctcgcccgct cccctgggct cagctcggac gctgcagtta ttgacctccc
1441 ggtcccgcct gcccgccctc ccccacgtgg ccccttgacc cgggcggccc cgccgcttct
1501 ttccttcctg cagttcccag ccctcggagc ccccatccct tatcttaccc ccaccgcgct
1561 cccccaggag cgctccctca gctctctcct catccatcac cagtggagtt tttttatttt
1621 ttattttttt aaaagtttag gtgcctttgc ggatgacctc attttgacgt tgaaaaaatg
1681 attttttaat atgtgaacac tgcaaaaatg tgtttaaatt atctttttta aaacctattc
1741 aggattatta gcctggactt ggacacagag tttgtaaata aaggtgtctg tgcagatttt
1801 cccactgatt tatttgtata aaaatactca tcttttcaga cttttttgta aacccccagt
1861 tgtgaaaact gcagtttagc agtgacctca gcaacccctc ctttttattt tttcctttaa
1921 aaacatttca gttaaattaa gctactgatt tggatttgtt ttatcgtatc ctaaagtctt
1981 tgttgttgaa atgaaaggta ttttggggtt atttattatg aaaacaacat gctcttaatg
2041 ttgattttac aatatgaaga gattatttaa ataaattatt gttttcattg g
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下游引物 5-CACCAAAGTCCCAGGAGGCCG-3
1 cggcctcaac aacgtccctt actccgccaa gagcagctat gacctgggct acaccgccgc
61 ctacacctcc tacgctccct atggaaccag ttcgtcccca gccaacaacg agcctgagaa
121 ggaggacctt gagcctgaaa ttcggatagt gaacgggaag ccaaagaaag tccggaaacc
181 ccgcaccatc tactccagtt tccagctggc ggctcttcag cggcgtttcc aaaagactca
241 gtacttggcc ttgccggagc gagccgagct ggcggcctct ctgggcctca cccagactca
301 ggtcaaaatc tggttccaga accgccggtc caagttcaag aagatgtgga aaagtggtga
361 gatcccctcg gagcagcacc ctggggccag cgcttctcca ccttgtgctt cgccgccagt
421 ctcagcgccg gcctcctggg actttggtgt gccgcagcgg atggcgggcg gcggtggtcc
481 gggcagtggc ggcagcggcg ccggcagctc gggctccagc ccgagcagcg cggcctcggc
541 ttttctgggc aactacccct ggtaccacca gacctcggga tccgcctcac acctgcaggc
601 cacggcgccg ctgctgcacc ccactcagac cccgcagccg catcaccacc accaccatca
661 cggcggcggg ggcgccccgg tgagcgcggg gacgattttc taa
1 atgactggag tctttgacag tctagtggct gatatgcact cgacccagat cgccgcctcc
61 agcacgtacc accagcacca gcagcccccg agcggcggcg gcgccggccc gggtggcaac
121 agcagcagca gcagcagcct ccacaagccc caggagtcgc ccacccttcc ggtgtccacc
181 gccaccgaca gcagctacta caccaaccag cagcacccgg cgggcggcgg cggcggcggg
241 ggctcgccct acgcgcacat gggttcctac cagtaccaag ccagcggcct caacaacgtc
301 ccttactccg ccaagagcag ctatgacctg ggctacaccg ccgcctacac ctcctacgct
361 ccctatggaa ccagttcgtc cccagccaac aacgagcctg agaaggagga ccttgagcct
421 gaaattcgga tagtgaacgg gaagccaaag aaagtccgga aaccccgcac catctactcc
481 agtttccagc tggcggctct tcagcggcgt ttccaaaaga ctcagtactt ggccttgccg
541 gagcgagccg agctggcggc ctctctgggc ctcacccaga ctcaggtcaa aatctggttc
601 cagaaccgcc ggtccaagtt caagaagatg tggaaaagtg gtgagatccc ctcggagcag
661 caccctgggg ccagcgcttc tccaccttgt gcttcgccgc cagtctcagc gccggcctcc
721 tgggactttg gtgtgccgca gcggatggcg ggcggcggtg gtccgggcag tggcggcagc
781 ggcgccggca gctcgggctc cagcccgagc agcgcggcct cggcttttct gggcaactac
841 ccctggtacc accagacctc gggatccgcc tcacacctgc aggccacggc gccgctgctg
901 caccccactc agaccccgca gccgcatcac caccaccacc atcacggcgg cgggggcgcc
961 ccggtgagcg cggggacgat tttctaaccc cagggagaac tcgccagaga ctgagagcag
1021 agaccactta tcctcattgc ttaccccgag ccggggttcc ctcctcccgg cccgctgccg
1081 ccacccacct ctcctgcagg ctgcgacctg cagtggcccg tctcaggccc tgctcactcc
1141 cggggccacc aaacgggccc ctctctcggg ggaaccggac agcagcttgg caaaggcctc
1201 cctaaaaggc cgcatttctg acctgagccc cgggtctcgg ctgtttcgag ccccgcctcg
1261 gacttgcctt ccctcccctc cgggtgagcc tgtctggcgc cttcctcgcc ccgggctgag
1321 agctgggtcc cgggagatgg aagcctccca ggcgcgcgag gcttcccggg cgctctgagg
1381 cttctttctc ctcgcccgct cccctgggct cagctcggac gctgcagtta ttgacctccc
1441 ggtcccgcct gcccgccctc ccccacgtgg ccccttgacc cgggcggccc cgccgcttct
1501 ttccttcctg cagttcccag ccctcggagc ccccatccct tatcttaccc ccaccgcgct
1561 cccccaggag cgctccctca gctctctcct catccatcac cagtggagtt tttttatttt
1621 ttattttttt aaaagtttag gtgcctttgc ggatgacctc attttgacgt tgaaaaaatg
1681 attttttaat atgtgaacac tgcaaaaatg tgtttaaatt atctttttta aaacctattc
1741 aggattatta gcctggactt ggacacagag tttgtaaata aaggtgtctg tgcagatttt
1801 cccactgatt tatttgtata aaaatactca tcttttcaga cttttttgta aacccccagt
1861 tgtgaaaact gcagtttagc agtgacctca gcaacccctc ctttttattt tttcctttaa
1921 aaacatttca gttaaattaa gctactgatt tggatttgtt ttatcgtatc ctaaagtctt
1981 tgttgttgaa atgaaaggta ttttggggtt atttattatg aaaacaacat gctcttaatg
2041 ttgattttac aatatgaaga gattatttaa ataaattatt gttttcattg g
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