The E.Z.N.A.® Mag-Bind Dye terminator Removal Procedure
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实验试剂
1. Absolute (96%-100%) ethanol
2. Molecular biology grade water
实验设备
3. Magnetic Separation Devices
实验步骤
1. Determine the volume of the crude sequencing product and transfer the sample into 96-well microplate. Add 10 ul molecular grade water to each sample.
2. Add 2 volume of Mag-Bind® Particle/ethanol mixture and mix thoroughly by pipetting.
3. Incubate 10 minutes at room temperature. Place the microplate on a magnetic separation device designed for 96-well plate. Wait for 5 minute or until the solution is clear of beads.
4. Aspirate entire solution with multiple channel pipettor. Do not disturb the Mag-Bind™ Particle pellet.
5. Dispense 100ul MPG Wash Buffer into each well, resuspend Mag-Bind Particle pellet by pipetting. Incubate for 1 minute at room temperature.
6. Place the microplate on a magnetic separation device, and wait 1 minute or until the solution is clear of beads.
7. Aspirate entire solution with a multiple channel pipettor. Do not disturb the Mag-Bind™ Particle pellet.
8. Dry the Mag-Bind™Particle pellet by air for 10 minutes.
9. Remove the plate from separation device. Add 15ul - 30ul water to each sample and resuspend Mag-Bind® Particle pellet by pipetting.
10. Incubate 10 minutes at room temperature. Place the microplate on a magnetic separation device designed for 96-well plate. Wait for 1 minute or until the solution is clear of beads.
11. Transfer the cleared supernatant to a new microplate (not provided).
