E.Z.N.A. Mag-Bind Blood DNA Protocol (1-100 ul Blood)
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实验试剂
Regents to be provided by user
1. Absolute ethanol (96%-100%)
实验设备
Equipments to be provided by user
1. Nuclease-free 1.5 mL centrifuge tube (for single tube preparation)
2. 500 ul Nuclease-free microplate
3. 1.2mL deep well plate (EV1772)
4. 8-strip caps for 1.2mL deep well plate (EV1774)
5. Water bath, incubator or heating block preset at 65° C
6. Magnetic separation device for microcentrifuge tubes or microplates
实验步骤
3. Incubate sample at 65°C for 15 min. Briefly vortexing the tube during incubation.
8. Resuspend the Mag-Bind particles pellet by vortexing at maxi speed for 30 seconds.
11. Resuspend the Mag-Bind particles pellet by vortexing at maxi speed for 30 seconds.
14. Resuspend the Mag-Bind particles pellet by vortexing at maxi speed for 30 seconds.
19. Place the tube onto a magnetic separation device to magnetize the Mag-Bind particles.
20. Transfer the cleared supernatant containing purified DNA to a new 1.5 mL tube or plate.