Dynabeads® DNA DIRECT Blood
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实验试剂
Dynal® magnet: see www.invitrogen.com/magnets-selection.
Sterile, round-bottomed 2 ml microcentrifuge tubes.
Micropipettors and sterile, disposable pipette tips.
65°C water-bath or heating block (only needed for elution).
PBS pH 7.4 (Phosphate Buffered Saline - for buffy coat, see below).
Sterile and PCR-grade water for dilution of supplied buffers.
实验步骤
1. DNA Isolation Protocol from 100 μl Blood
5) Leave tube undisturbed at room temperature for 5 minutes.
2. DNA Isolation Protocol from 500 μl Blood
1) Take 0.5 ml anti-coagulated whole blood in a 2 ml round bottomed microcentrifuge tube.
9) Replace the magnetic field and after 30 seconds, aspirate and discard the supernatant.