Expression of NMDA Receptor Channel Subunit Proteins Using Baculovirus and Herpesvirus Vectors

互联网2014-02-14

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Foreign gene transfer and expression in the cells are among the most important techniques for determining the characteristics and function of cloned genes. Glutamate receptor genes have been expressed by RNA injection of Xenopus oocytes or transfection of mammalian cells, such as HEK, CHO, and COS cells, and bacterial Escherichia coli cells. Viral vectors, such as baculovirus (Autographa californica nuclear polyhedrosis virus, AcNPV) or herpesvirus (herpes simplex virus-1, HSV-1), have been also used because of high efficiency of gene transfer. Existing viral clones expressing recombinant glutamate receptor proteins (1 –25 ) are summarized in Table 1 .

Table 1 Summary of Viral Vectors Expressing Glutamate Receptor Proteins

Viral vector, mouseGluR/ratGluR	Reference
Baculovirus vector
α1	*1–5*
α2/GluRB	*3,6–9*
(α4)/GluRD	*9–11*
(β2)/GluR6	*12–14*
ζ1/NMDAR1	*15–17*
ε1	18 and this study
ε2	18 and this study
δ2	18 and this study
mGluR1 (rat)	13
Herpesvirus vector
(α1)/GluR1	19
(β2)/GluR6	*20–22*
ζ1	This study
Adenovirus vector
(a1)/GluR1	23
(a2)/GluR2	23
(ζ1)/NMDAR1 (antisense)	24
Adenoassociated virus vector
(ζ1)/NMDAR1 (antisense)	25

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