SDS-PAGE Solutions

 

Stacking Gel

	7%	10%
4x stacking gel buffer	25 ml	25 ml
30% acrylamide stock	23.3 ml	33.3 ml
20% SDS	0.5 ml	0.5 ml
ddH2O	51.2 ml	41.2 ml

                              

 

4x Stacking Gel Buffer

0.5 M Tris-HCl 7.9 g/100 ml (pH 6.8)

Resolving Gel

200 mls

 

                  
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4x Resolving Gel Buffer

1.5 M Tris-base 18.2 g/100 ml (pH 8.8)

Running Buffer (10 L) - do not adjust the pH

Tris-Base  30 g
Glycine 144 g
20% SDS 50 mls
Fill to 10 L

Acrylamide Stock (30% acrylamide - 0.8% bis-acrylamide) - 500 mls

acrylamide - 150 g
bis-acrylamide - 4 g
make to 500 mls and filter through a 0.45 um filter

SDS-PAGE 3X Sample Buffer (10 ml)

125 mM Tris-HCl  2.5 ml of 4X Stacking Gel Buffer
6% SDS  3.0 ml of 20% SDS
6% B-ME  600 ul
30% glycerol  3.0 ml
ddH2O   900 ul