PCR引物设计
丁香园论坛
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我设计了一对引物，用几个软件评价不一，哪位高手能否帮我检测一下？PA:ATCAAGCTTCACAGTCAGCCGCATGG
PB:GCTTCTAGACCTCTGGCTGGCTTCTCAC
我的序列：gcccgtacac accgtgtgct gggacacccc acagtcagcc gcatggctcc cctgtgcccc
61 agcccctggc tccctctgtt gatcccggcc cctgctccag gcctcactgt gcaactgctg
121 ctgtcactgc tgcttctgat gcctgtccat ccccagaggt tgccccggat gcaggaggat
181 tcccccttgg gaggaggctc ttctggggaa gatgacccac tgggcgagga ggatctgccc
241 agtgaagagg attcacccag agaggaggat ccacccggag aggaggatct acctggagag
301 gaggatctac ctggagagga ggatctacct gaagttaagc ctaaatcaga agaagagggc
361 tccctgaagt tagaggatct acctactgtt gaggctcctg gagatcctca agaaccccag
421 aataatgccc acagggacaa agaaggggat gaccagagtc attggcgcta tggaggcgac
481 ccgccctggc cccgggtgtc cccagcctgc gcgggccgct tccagtcccc ggtggatatc
541 cgcccccagc tcgccgcctt ctgcccggcc ctgcgccccc tggaactcct gggcttccag
601 ctcccgccgc tcccagaact gcgcctgcgc aacaatggcc acagtgtgca actgaccctg
661 cctcctgggc tagagatggc tctgggtccc gggcgggagt accgggctct gcagctgcat
721 ctgcactggg gggctgcagg tcgtccgggc tcggagcaca ctgtggaagg ccaccgtttc
781 cctgccgaga tccacgtggt tcacctcagc accgcctttg ccagagttga cgaggccttg
841 gggcgcccgg gaggcctggc cgtgttggcc gcctttctgg aggagggccc ggaagaaaac
901 agtgcctatg agcagttgct gtctcgcttg gaagaaatcg ctgaggaagg ctcagagact
961 caggtcccag gactggacat atctgcactc ctgccctctg acttcagccg ctacttccaa
1021 tatgaggggt ctctgactac accgccctgt gcccagggtg tcatctggac tgtgtttaac
1081 cagacagtga tgctgagtgc taagcagctc cacaccctct ctgacaccct gtggggacct
1141 ggtgactctc ggctacagct gaacttccga gcgacgcagc ctttgaatgg gcgagtgatt
1201 gaggcctcct tccctgctgg agtggacagc agtcctcggg ctgctgagcc agtccagctg
1261 aattcctgcc tggctgctgg tgacatccta gccctggttt ttggcctcct ttttgctgtc
1321 accagcgtcg cgttccttgt gcagatgaga aggcagcaca gaaggggaac caaagggggt
1381 gtgagctacc gcccagcaga ggtagccgag actggagcct agaggctgga tcttggagaa
1441 tgtgagaagc cagccagagg catctgaggg ggagccggta actgtcctgt cctgctcatt
1501 atgccacttc cttttaactg ccaagaaatt ttttaaaata aatatttata at
谢谢
PB:GCTTCTAGACCTCTGGCTGGCTTCTCAC
我的序列：gcccgtacac accgtgtgct gggacacccc acagtcagcc gcatggctcc cctgtgcccc
61 agcccctggc tccctctgtt gatcccggcc cctgctccag gcctcactgt gcaactgctg
121 ctgtcactgc tgcttctgat gcctgtccat ccccagaggt tgccccggat gcaggaggat
181 tcccccttgg gaggaggctc ttctggggaa gatgacccac tgggcgagga ggatctgccc
241 agtgaagagg attcacccag agaggaggat ccacccggag aggaggatct acctggagag
301 gaggatctac ctggagagga ggatctacct gaagttaagc ctaaatcaga agaagagggc
361 tccctgaagt tagaggatct acctactgtt gaggctcctg gagatcctca agaaccccag
421 aataatgccc acagggacaa agaaggggat gaccagagtc attggcgcta tggaggcgac
481 ccgccctggc cccgggtgtc cccagcctgc gcgggccgct tccagtcccc ggtggatatc
541 cgcccccagc tcgccgcctt ctgcccggcc ctgcgccccc tggaactcct gggcttccag
601 ctcccgccgc tcccagaact gcgcctgcgc aacaatggcc acagtgtgca actgaccctg
661 cctcctgggc tagagatggc tctgggtccc gggcgggagt accgggctct gcagctgcat
721 ctgcactggg gggctgcagg tcgtccgggc tcggagcaca ctgtggaagg ccaccgtttc
781 cctgccgaga tccacgtggt tcacctcagc accgcctttg ccagagttga cgaggccttg
841 gggcgcccgg gaggcctggc cgtgttggcc gcctttctgg aggagggccc ggaagaaaac
901 agtgcctatg agcagttgct gtctcgcttg gaagaaatcg ctgaggaagg ctcagagact
961 caggtcccag gactggacat atctgcactc ctgccctctg acttcagccg ctacttccaa
1021 tatgaggggt ctctgactac accgccctgt gcccagggtg tcatctggac tgtgtttaac
1081 cagacagtga tgctgagtgc taagcagctc cacaccctct ctgacaccct gtggggacct
1141 ggtgactctc ggctacagct gaacttccga gcgacgcagc ctttgaatgg gcgagtgatt
1201 gaggcctcct tccctgctgg agtggacagc agtcctcggg ctgctgagcc agtccagctg
1261 aattcctgcc tggctgctgg tgacatccta gccctggttt ttggcctcct ttttgctgtc
1321 accagcgtcg cgttccttgt gcagatgaga aggcagcaca gaaggggaac caaagggggt
1381 gtgagctacc gcccagcaga ggtagccgag actggagcct agaggctgga tcttggagaa
1441 tgtgagaagc cagccagagg catctgaggg ggagccggta actgtcctgt cctgctcatt
1501 atgccacttc cttttaactg ccaagaaatt ttttaaaata aatatttata at
谢谢
