Brdu免疫组织化学染色分析 BrdU incorporation assay

互联网2013-09-06

7164

Enzyme Immunostaining for BrdU:

2. Wash frozen sections with PBS 2x.
4. Put them in 0.1% Pepsin in 0.1N HCL (in PBS) at 37C for 50min.
6. Then in 0.3% hydrogen peroxide in PBS, 30min. Then rinse with PBS 3x.
8. Then in 5% normal goat serum in PBS with 1% BSA at RT for 1 hour.
10. Mouse anti-BrdU diluted to 1:1000 with 1% NGS and1%BSA in PBS, incubateat 4C overnight.
12. Rinse with PBS 3x.
14. Goat Biotinylated anti-Mouse IgG 1:200 in 1% NGS, 1%BSA in PBS, incubate at RT for 1 hour. Rinse in PBS 3x.
16. Incubate with ABC reagent in PBS containing 0.1% Tween 20 at RT for 1 hour.. (ABC reagent solution: 5ml PBS/0.1% Tween 20 with 1 drop A and 1 drop B, mix well).
18. Rinse with PBS 3x.
20. DAB reaction: 2.5ml of distilled water + 1 drop Buffer + 2 drop DAB solution + 1 drop hydrogen peroxide. ~Needs about 8 min to stop DAB reaction.
22. Rinse with distilled water 3x. Counterstain and mount.

Culture Cell Fluorescence Immunostaining for BrdU:

2. Wash plates with PBS 3x.
4. Fix them in 4%PFA, 20 min at RT.
6. Wash them in PBS 3x.
8. 0.5% Triton X-100 in PBS, 15min at 4C
10. Wash slides in PBS 3x.
12. 2N HCL( in Distilled water) 30min at 37C( DNA denaturation).
14. Wash in PBS 5x
16. Incubate slides with 1%BSA in PBS at RT for 30min.
18. Mouse anti-BrdU diluted to 1:1000 with 1% NDS and1%BSA in PBS, incubate at RT for 2hrs.
20. Wash with PBS 3x.
22. 1: 100 dilution of Rhodamine(Tritc) conjugated Donkey anti mouse IgG with 1% NDS,1%BSA in PBS, incubate at RT for 1 hr.
24. Wash in PBS 3x
26. Mount with 50% glycerol in PBS.

Culture Cell Enzyme Immunostaining for BrdU:

相关产品推荐

外泌体研究（提取、鉴定、分析等）及外泌体工程化改造

询价

的BCIP/NBT显色底物试剂盒包含用于显色印迹和免疫组织化学染色应用的现成溶液|s BCIP/ NBT Chromogenic Substrate Kit contains ready-made solutions for chromogenic blot and immunohistochemical staining applications.

￥11

DiR' [DiIC18(7)],用于膜染色，100068-60-8，阿拉丁

￥369.90

BrdU Mouse mAb，阿拉丁

￥199.90

Firefly Luciferase Assay Kit（萤火虫萤光素酶报告基因检测试剂盒），阿拉丁

￥570.90