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         Tips and hints for the storage of antibodies (Synaptic Systems)

         Purification of IgG Using Protein A- or Protein G-Agarose (KPL)

         Production  of Monoclonal Antibody (Chisholm Lab)
Protocol for making monoclonal antibodies. All steps of the entail procedure are described in great details

         Production of Monoclonal Antibody in Mice   (Walter Steffen)
Whole procedures for monoclonal antibody production

         Preparation of Monoclonal Antibody (PMCI)
Protocol for making hybridoma and antibody

  • Cell Fusion and Selection (PMCI)
  • Fusion of Mouse, Rat, or Hamster Cell (Contributed by Nanci Donacki)
      
  • Fusion and Cloning (Contributed by Nanci Donacki)
    Procedure for establishing hybridoma in one step
      
  • Cloning by Limiting Dilution (Contributed by Nanci Donacki)
      

         Affinity Purification of Monoclonal Antibodies From Tissue (PMCI)
  

         Expanding Hybridoma Clones (PMCI)
   

         Protein G Purification of Antibody (Contributed by Nanci Donacki)
  

         Protein A Purification of Antibody (Contributed by Nanci Donacki)

 

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