Preparation of cell lysates from E. coli by enzymatic lysis
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Preparation of cell lysates from E. coli by enzymatic lysis
Materials
Chemicals
| lysosyme |
Lysis buffer
| <center> <font>50 mM Tris-HCl pH 7.5 </font> <p> <font>50-200 mM NaCundefined</font></p> <p> <font>5% glycerol (v/v)</font></p> <p> <font>1 mM DTT</font></p> <p> <font>1 mM PMSF</font></p> </center> |
