[旧贴整理]提取RNA,以及RT-PCR的问题整理
丁香园论坛
1133
1。提取RNA的原理
http://www.dxy.cn/bbs/actions/archive/post/2476706_0.html
2。细胞RNA的提取
(1)提取RNA要求的细胞数
http://www.dxy.cn/bbs/actions/archive/post/72002_0.html
http://www.dxy.cn/bbs/actions/archive/post/2860126_0.html
http://www.dxy.cn/bbs/actions/archive/post/2122999_0.html
http://www.dxy.cn/bbs/post/view?bid=66&id=3031023&sty=3&keywords=%CC%E1%C8%A1RNA
(2)提取RNA的干燥问题
http://www.dxy.cn/bbs/thread/4096049
(3)冻存细胞RNA的提取
http://www.dxy.cn/bbs/actions/archive/post/112830_0.html
3.提取组织RNA
(1)提取RNA组织的需要量
http://www.dxy.cn/bbs/actions/archive/post/1033424_0.html
(2)如何消化组织块以及研磨
http://www.dxy.cn/bbs/thread/4487736
http://www.dxy.cn/bbs/actions/archive/post/311885_0.html
(3)提取组织RNA的步骤以及取材
http://www.dxy.cn/bbs/actions/archive/post/2909649_0.html
http://www.dxy.cn/bbs/actions/archive/post/2209521_0.html
http://www.dxy.cn/bbs/post/view?bid=64&id=3445976&sty=3&keywords=%CC%E1%C8%A1RNA
(4)提取组织RNA的试剂盒推荐
http://www.dxy.cn/bbs/thread/3710074
4。植物RNA提取
http://www.dxy.cn/bbs/thread/4547511
5。血清(血液)提取RNA
http://www.dxy.cn/bbs/actions/archive/post/2708153_0.html
http://www.dxy.cn/bbs/post/view?bid=67&id=4378399&sty=3&keywords=%CC%E1%C8%A1RNA
6。提取RNA的器械以及DEPC水准备
http://www.dxy.cn/bbs/actions/archive/post/3220624_0.html
http://www.dxy.cn/bbs/actions/archive/post/3232700_0.html
http://www.dxy.cn/bbs/post/view?bid=67&id=4274141&sty=3&keywords=%CC%E1%C8%A1RNA
http://www.dxy.cn/bbs/thread/3096928
7。提取RNA的实际操作
http://www.dxy.cn/bbs/thread/4449685(多加入TRIZOL是否有问题)
http://www.dxy.cn/bbs/actions/archive/post/2115912_0.html
http://www.dxy.cn/bbs/actions/archive/post/2954276_0.html
http://www.dxy.cn/bbs/actions/archive/post/804421_0.html
http://www.dxy.cn/bbs/post/view?bid=67&id=536446&sty=3&keywords=%CC%E1%C8%A1RNA
8。RNA电泳分析
http://www.dxy.cn/bbs/post/view?bid=154&id=4473553&sty=3&keywords=%CC%E1%C8%A1RNA
http://www.dxy.cn/bbs/actions/archive/post/2563841_0.html
9。提取RNA的注意事项
http://www.dxy.cn/bbs/actions/archive/post/3594229_0.html
http://www.dxy.cn/bbs/actions/archive/post/2655507_0.html
10。提取RNA后如何在剩余相中提取蛋白质,DNA
http://www.dxy.cn/bbs/thread/4297529
http://www.dxy.cn/bbs/actions/archive/post/2696524_0.html
11.RT-PCR试剂盒推荐
http://www.dxy.cn/bbs/actions/archive/post/1960541_0.html
12。RT-PCR的步骤
http://www.dxy.cn/bbs/post/view?bid=64&id=378657&sty=3&keywords=RT-PCR
http://www.dxy.cn/bbs/actions/archive/post/83473_0.html
13RT-PCR讨论
http://www.dxy.cn/bbs/actions/archive/post/83473_0.html
http://www.dxy.cn/bbs/actions/archive/post/132275_0.html
http://www.dxy.cn/bbs/actions/archive/post/2476706_0.html
2。细胞RNA的提取
(1)提取RNA要求的细胞数
http://www.dxy.cn/bbs/actions/archive/post/72002_0.html
http://www.dxy.cn/bbs/actions/archive/post/2860126_0.html
http://www.dxy.cn/bbs/actions/archive/post/2122999_0.html
http://www.dxy.cn/bbs/post/view?bid=66&id=3031023&sty=3&keywords=%CC%E1%C8%A1RNA
(2)提取RNA的干燥问题
http://www.dxy.cn/bbs/thread/4096049
(3)冻存细胞RNA的提取
http://www.dxy.cn/bbs/actions/archive/post/112830_0.html
3.提取组织RNA
(1)提取RNA组织的需要量
http://www.dxy.cn/bbs/actions/archive/post/1033424_0.html
(2)如何消化组织块以及研磨
http://www.dxy.cn/bbs/thread/4487736
http://www.dxy.cn/bbs/actions/archive/post/311885_0.html
(3)提取组织RNA的步骤以及取材
http://www.dxy.cn/bbs/actions/archive/post/2909649_0.html
http://www.dxy.cn/bbs/actions/archive/post/2209521_0.html
http://www.dxy.cn/bbs/post/view?bid=64&id=3445976&sty=3&keywords=%CC%E1%C8%A1RNA
(4)提取组织RNA的试剂盒推荐
http://www.dxy.cn/bbs/thread/3710074
4。植物RNA提取
http://www.dxy.cn/bbs/thread/4547511
5。血清(血液)提取RNA
http://www.dxy.cn/bbs/actions/archive/post/2708153_0.html
http://www.dxy.cn/bbs/post/view?bid=67&id=4378399&sty=3&keywords=%CC%E1%C8%A1RNA
6。提取RNA的器械以及DEPC水准备
http://www.dxy.cn/bbs/actions/archive/post/3220624_0.html
http://www.dxy.cn/bbs/actions/archive/post/3232700_0.html
http://www.dxy.cn/bbs/post/view?bid=67&id=4274141&sty=3&keywords=%CC%E1%C8%A1RNA
http://www.dxy.cn/bbs/thread/3096928
7。提取RNA的实际操作
http://www.dxy.cn/bbs/thread/4449685(多加入TRIZOL是否有问题)
http://www.dxy.cn/bbs/actions/archive/post/2115912_0.html
http://www.dxy.cn/bbs/actions/archive/post/2954276_0.html
http://www.dxy.cn/bbs/actions/archive/post/804421_0.html
http://www.dxy.cn/bbs/post/view?bid=67&id=536446&sty=3&keywords=%CC%E1%C8%A1RNA
8。RNA电泳分析
http://www.dxy.cn/bbs/post/view?bid=154&id=4473553&sty=3&keywords=%CC%E1%C8%A1RNA
http://www.dxy.cn/bbs/actions/archive/post/2563841_0.html
9。提取RNA的注意事项
http://www.dxy.cn/bbs/actions/archive/post/3594229_0.html
http://www.dxy.cn/bbs/actions/archive/post/2655507_0.html
10。提取RNA后如何在剩余相中提取蛋白质,DNA
http://www.dxy.cn/bbs/thread/4297529
http://www.dxy.cn/bbs/actions/archive/post/2696524_0.html
11.RT-PCR试剂盒推荐
http://www.dxy.cn/bbs/actions/archive/post/1960541_0.html
12。RT-PCR的步骤
http://www.dxy.cn/bbs/post/view?bid=64&id=378657&sty=3&keywords=RT-PCR
http://www.dxy.cn/bbs/actions/archive/post/83473_0.html
13RT-PCR讨论
http://www.dxy.cn/bbs/actions/archive/post/83473_0.html
http://www.dxy.cn/bbs/actions/archive/post/132275_0.html