E.Z.N.A.TM Fast Protocol For FFPE Tissue
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实验原理
实验步骤
3. Incubate at 80°C for 15 min. Incubate for 1 minutes at room temperature.
4. Immediately add 20 ul OB Protease to the tissue, mix by vortexing.
5. Incubate at 55°C for 15-60 min.
8. Add 220 ul Buffer GTC and vortex to mix.
9. Add 660 ul absolute ethanol and mix thoroughly by vortexing.
15. To elute RNA from the column, centrifuge at 10,000 x g for 1 min.