DOP-PCR Labeling for Spectral Karyotyping (SKY)
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2465
Reagents
Agarose, Ultrapure
Ampli Taq polymerase 5 U/µl
Buffer, 10X and 25 mM MgCl 2
Loading buffer, 5X
Ethidium Bromide
Primer “UN1”
10X TAE buffer
Template DNA
Use 400 – 600 ng for each reaction
100 mM dNTP nucleotides
x – dUTP
Materials and Equipment
PCR Thermocycler
MJ – Research, Inc., Model PTC - 100
Gel system and power source
PCR tubes
PGC Scientifics, Cat. 502-075
