Hybridization for Spectral Karyotyping (SKY)

Reagents

dH 2O
Ethanol, absolute (70%, 90%, and 100%)
Formamide, deionized
Ambion, Cat. No. 9342
HCL, 1N
Rubber Cement
SSC, 20X

Preparation

70% Formamide/2X SSC
20X SSC 10 ml
dH 2O 20 ml
deionized formamide 70 ml
Adjust to pH 7.0 with 1N HCL
Aliquot and store at –20°C.
Pre-cool 70% ethanol to 0°C