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Antibody-Guided Selection Using Capture-Sandwich ELISA

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Antibody (Ab) phage display is a recently developed recombinant DNA technology for making human monoclonal antibodies (MAbs) from immune sources, such as bone marrow, lymph node, or peripheral blood lymphocytes from patients with various diseases, or from healthy individuals (1,2). Many human MAb Fabs or scFvs specific for viral pathogens, self antigens (Ags), or nonself Ags have been isolated by phage display system. This technology is expected to provide more powerful diagnostic, prophylactic, and therapeutic tools of human origin than do currently used polyclonal Abs or MAbs derived from other species.

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