Cloning Genes from T-DNA Tagged Mutants

Insertion mutagenesis has a major advantage over other types of mutagenesis in that it not only causes a disruption of the gene, but the insertion element serves as a vehicle for recovery of the flanking DNA. Two types of insertion mutagens used extensively in plants are T-DN As and transposons. Each of these mutagens has then own advantages and disadvantages. A few of the advantages of T-DNAs are that they are: