切除融合标签常用蛋白酶的酶切位点

互联网2008-07-21

25883

Cleavage Sites Table for Fusion Proteins：

PROTEASE	COMPANY	Protease Capture
Thrombin Leu-Val-Pro-Arg�Gly-Ser	Amersham-Biosciences, Novagen, SIGMA, Roche	Benzamidine-Agarose
Factor Xa Ile-Glu/Asp-Gly-Arg�	Amersham-Biosciences, New England Biolabs, Roche	Benzamidine-Agarose
Enterokinase Asp-Asp-Asp-Asp-Lys�	New England Biolabs, Novagen, Roche	Trypsin Inhibitor-Agarose
TEV protease Glu-Asn-Leu-Tyr-Phe-Gln�Gly	Invitrogen – Life Technologies	Ni-NTA (6His recomb. TEV)
PreScission Leu-Glu-Val-Leu-Phe-Gln�Gly-Pro	Amersham-Biosciences	GSTrap for GST fusion enzyme
TAGZyme His-tag removal by Exoproteolytic Digestion	Qiagen	Ni-NTA (6His recomb. enzyme)
Intein Site dithiothreitol cleavage	New England Biolabs	DTT elimination by dialysis
HRV 3C Protease Leu-Glu-Val-Leu-Phe-Gln�Gly-Pro	Novagen	Ni-NTA (6His recomb. enzyme)
SUMO Protease recognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMO	Invitrogen – Life Technologies, LifeSensors	Ni-NTA (6His recomb. enzyme)