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切除融合标签常用蛋白酶的酶切位点

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Cleavage Sites Table for Fusion Proteins:

PROTEASE
COMPANY
Protease Capture
Thrombin
Leu-Val-Pro-Arg�Gly-Ser
Amersham-Biosciences, Novagen,
SIGMA, Roche
Benzamidine-Agarose
Factor Xa
Ile-Glu/Asp-Gly-Arg�
Amersham-Biosciences, New England Biolabs,
Roche
Benzamidine-Agarose
Enterokinase
Asp-Asp-Asp-Asp-Lys�
New England Biolabs,
Novagen, Roche
Trypsin Inhibitor-Agarose
TEV protease
Glu-Asn-Leu-Tyr-Phe-Gln�Gly
Invitrogen – Life Technologies
Ni-NTA (6His recomb. TEV)
PreScission
Leu-Glu-Val-Leu-Phe-Gln�Gly-Pro
Amersham-Biosciences
GSTrap for GST fusion enzyme
TAGZyme
His-tag removal by Exoproteolytic Digestion
Qiagen
Ni-NTA (6His recomb. enzyme)
Intein Site
dithiothreitol cleavage
New England Biolabs
DTT elimination by dialysis
HRV 3C Protease
Leu-Glu-Val-Leu-Phe-Gln�Gly-Pro
Novagen
Ni-NTA (6His recomb. enzyme)
SUMO Protease
recognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMO
Invitrogen – Life Technologies, LifeSensors
Ni-NTA (6His recomb. enzyme)

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